Macrophage Scavenger Receptor 1 antibody (FITC)

Catalog No. ABIN319577

The Rat Monoclonal anti-Macrophage Scavenger Receptor 1 antibody has been validated for FACS. It is suitable to detect Macrophage Scavenger Receptor 1 in samples from Mouse.

Quick Overview for Macrophage Scavenger Receptor 1 antibody (FITC) (ABIN319577)

Target: Macrophage Scavenger Receptor 1 (MSR1)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Macrophage Scavenger Receptor 1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 2F8

Product Details anti-Macrophage Scavenger Receptor 1 Antibody (FITC)

Specificity: This antibody recognises the Murine Scavenger Receptor class A (SRA), type I and II, also known as CD204. Clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000).

Purification: Affinity Chromatography on Protein G.

Immunogen: RAW264 cell line. Remarks: Spleen cells from immunised AO rats were fused with cells of the Y3 rat myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Macrophage Scavenger Receptor 1

Target: Macrophage Scavenger Receptor 1 (MSR1)

Alternative Name: CD204

Background: CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion. Recent research shows that clone 2F8 recognises an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain.Synonyms: MSR1, Macrophage acetylated LDL receptor I and II, Macrophage scavenger receptor types I and II, SCARA1, Scavenger receptor class A member 1

Gene ID: 20288

NCBI Accession: NP_001106797

UniProt: P30204